anti human mouse nhp pstat4  (Cell Signaling Technology Inc)

Journal: PLOS One

Article Title: The ubiquitin-like modifier FAT10 does not affect IL-12 expression and signaling

doi: 10.1371/journal.pone.0323005

Figure Lengend Snippet: (A-D) CD4 + T cells were magnetically purified from spleens of C57BL/6 wild type mice or FAT10 -/- mice. (A, B) CD4 + T cells were treated with TNF (400 U/ml)/IFN-γ (200 U/ml) (indicated +) for 1 day or were left untreated (indicated -). pSTAT1, STAT1, and GAPDH were analyzed by western blot. (B) Quantification of pSTAT1 normalized to GAPDH. Data are depicted as mean ± SDs derived from 7 different experiments (n = 7). (C, D) CD4 + T cells were treated with TNF (400U/ml)/IFN-γ (200 U/ml) for 1 day or left untreated, followed by a treatment with IL-12 (10 ng/ml) for 2 h. Treatment regime is indicated. pSTAT4 and GAPDH were analyzed by western blot. (D) Quantification of pSTAT4 normalized to GAPDH. Data are depicted as mean ± SDs derived from 5 different experiments (n = 5).

Article Snippet: Protein was analyzed by the following antibodies: anti-pSTAT1 antibody (Cell Signaling, 7649S), anti-STAT1 antibody (Cell Signaling, 9172S), and anti-pSTAT4 antibody (Cell Signaling, 4134S).

Techniques: Purification, Western Blot, Derivative Assay